A small multigene family encodes Gi signal-transduction proteins.
- 1 November 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (22), 7886-7890
- https://doi.org/10.1073/pnas.84.22.7886
Abstract
The guanine nucleotide-binding regulatory proteins known as G proteins are receptor-associated signal transduction molecules that are implicated in the control of a variety of metabolic processes. Recent evidence suggests that G proteins may mediate B-lymphocyte responses to bacterial lipopolysaccharide and may also transduce signals from the T-cell antigen receptor. Since these receptors are uniquely expressed on lymphoid cells, we used molecular cloning strategies to ask whether lymphocytes contain specialized G-protein .alpha. subunits to assist in signal transduction. Comparison of our two deduced human .alpha.i amino acid sequences with those previously determined for bovine and rodent G proteins permits the identification of three closely related but distinct types of .alpha.i molecules that comprise a small multigene family. Using gene-specific probes, we found that both of our .alpha.i genes are expressed in most cell types but in differing ratios. Our data support the view that a modest repertoire of extremely closely related G proteins mediates the transduction of signals derived from multiple different receptor molecules.Keywords
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