Protein synthesis by microsomal particles from regenerating rat liver

Abstract

Washed microsome particles from regenerating liver were shown to incorporate [14C]leucine into protein more actively than similar preparations from normal liver. The total incorporation in the preparations from regenerating liver increased linearly with the amount of protein incubated, whereas this was not so with preparations from normal liver. The greater activity of regenerating-liver microsomes appeared to be associated with the bound polysomes. The size distribution of polysomes obtained after removal of membrane with de-oxycholate was the same in normal and regenerating liver. In general the activity of polysome preparations from normal and regenerating liver was similar. The greater activity of the particles in the micro-some fraction from regenerating liver is to be attributed to the ribo-somes bound to membrane and that their activity is controlled by factors present in the membrane.