BIOSYNTHESIS OF SUGARS FOUND IN BACTERIAL POLYSACCHARIDES: PART II. BIOSYNTHESIS OFD-glycero-D-manno-HEPTOSE

Abstract
Azotobacter indicum was grown in media which contained D-glucose-1-C14, D-glucose-2-C14, D-glucose-6-C14, D-mannose-1-C14, or D-galactose-1-C14. Each radioactive polysaccharide produced was hydrolyzed and the specific activity of the isolated D-glycero-D-manno-heptose was determined. The distribution of activity in the heptose molecule was determined by chemical degradation procedures.The results suggest that the D-glycero-D-manno-heptose is formed from hexose precursors by pathways which involve the action of transaldolase, transketolase, and aldolase