EVIDENCE AGAINST PURINERGIC NERVE FIBRES IN THE HYPOGASTRIC NERVES OF THE CAT

Abstract

Hypogastric nerve stimulation (HGS) produced a transient contraction of the cat urinary bladder and inhibition of pelvic nerve evoked bladder contractions. Pretreatment by guanethidine (GUA) antagonized HGS inhibition of pelvic nerve evoked bladder contractions but had no effect on the HGS-induced transient contractions. GUA pretreatment had no effect on ATP or APPCP[.beta.-.gamma.-methylene ATP]-induced contractions of the urinary bladder. Pretreatment by 6-hydroxydopamine (6-OHDA) antagonized both the transient contraction and the inhibition of pelvic nerve evoked bladder contractions induced by HGS. 6-OHDA pretreatment had no effect on ATP or APPCP-induced contractions of the urinary bladder.