Conformation of N 6 -Methyladenine, a Base Involved in DNA Modification: Restriction Processes
- 23 November 1973
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 182 (4114), 833-834
- https://doi.org/10.1126/science.182.4114.833
Abstract
Crystal structures of N6,N9-dimethyladenine and N6-methyladenine hydrochloride were determined from three-dimensional x-ray diffraction data. The bases assume a conformation in which the N(6)-methyl group blocks one of the hydrogen-bonding sites normally used by adenine to form Watson-Crick pairs with thymine in double-helical DNA. When in this conformation, N6-methyladenine residues might alter the secondary structure of DNA. thereby preventing the scission of modified DNA's by restriction enzymes.Keywords
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