DNA Modification Methylase Activity of Escherichia coli Restriction Endonucleases K and P
- 1 November 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (11), 3138-3141
- https://doi.org/10.1073/pnas.69.11.3138
Abstract
The highly purified restriction endonucleases of E. coli K and coliphage P1 transfer methyl groups from S-adenosylmethionine to adenine residues of unmodified DNA. Incubation of unmodified DNA with endonucleases K or P and S-adenosylmethionine renders the DNA resistant to restriction. The enzymes, therefore, have both restriction endonuclease and modification methylase activities.Keywords
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