Method for Tissue Hemoglobin Analysis

Abstract

A colorimetric method for analysis of the red cell content of tissues is described, and compared with an independent radioassay method. The introduced method is based upon acid extraction of tissue, treatment with base, and adsorption of the remaining turbidity with lauryl sulfate. Preliminary data are presented on the reproducibility of the colorimetric method on multiple biopsies of the canine liver. Red cell content of biopsies obtained from a series of normal dogs was 0.031 ml./gm. of liver tissue with a standard deviation of ± 0.011. The red cell content of the liver of dogs in various physiologic conditions ranged up to 0.096 ml./gm. of liver tissue.