RNA folding causes secondary structure rearrangement
Open Access
- 29 September 1998
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 95 (20), 11555-11560
- https://doi.org/10.1073/pnas.95.20.11555
Abstract
The secondary structure of the P5abc subdomain (a 56-nt RNA) of the Tetrahymena thermophila group I intron ribozyme has been determined by NMR. Its base pairing in aqueous solution in the absence of magnesium ions is significantly different from the RNA in a crystal but is consistent with thermodynamic predictions. On addition of magnesium ions, the RNA folds into a tertiary structure with greatly changed base pairing consistent with the crystal structure: three Watson–Crick base pairs, three G⋅U base pairs, and an extra-stable tetraloop are lost. The common assumption that RNA folds by first forming secondary structure and then forming tertiary interactions from the unpaired bases is not always correct.Keywords
This publication has 25 references indexed in Scilit:
- Solution Conformation of a Five-Nucleotide RNA Bulge Loop from a Group I Intron,Biochemistry, 1997
- The structure of an RNA “kissing” hairpin complex of the HIV TAR hairpin loop and its complementJournal of Molecular Biology, 1997
- Control of Memory Formation Through Regulated Expression of a CaMKII TransgeneScience, 1996
- The Origin of the Great Bend of the Nile from SIR-C/X-SAR ImageryScience, 1996
- Metal-binding sites in the major groove of a large ribozyme domainStructure, 1996
- Thermal unfolding of a group I ribozyme: the low-temperature transition is primarily disruption of tertiary structureBiochemistry, 1993
- Visualizing the Higher Order Folding of a Catalytic RNA MoleculeScience, 1991
- Melting and chemical modification of a cyclized self-splicing group I intron: similarity of structures in 1 M sodium, in 10 mM magnesium and in the presence of substrateBiochemistry, 1990
- RNA pseudoknotsJournal of Molecular Biology, 1990
- Exchangeable proton NMR without base-line distorsion, using new strong-pulse sequencesJournal of the American Chemical Society, 1982