The genetic control of T cell‐mediated immunity against the DBA/2 mastocytoma P‐815. II. Low responsiveness in T cell‐mediated cytotoxicity accompanied by the inability to produce antibodies in a secondary response

Abstract

Low responsiveness of DBA/1 mice in T cell-mediated cytotoxicity against the DBA/2 mastocytoma P-815 (H-2^d) is genetically controlled and inherited as a recessive trait. For an analysis of the humoral anti-H-2^d response of DBA/1 mice after immunization with P-815 mastocytoma cells both complementdependent lysis (CDL) and the cytotoxicity inhibition assay (CIA) were used to demonstrate antibodies. Both assays show that DBA/1 mice are unable to produce anti-H-2^d antibodies in a secondary response. Even high doses of immunizing cells give only a weak response. is fully recovered upon allogeneic interaction. A transient primary anti-H-2^d activity can be found in the CIA but not by CDL. The data show that the genetically controlled determinant-specific defect in T cell-mediated cytotoxicity among DBA/1 mice has its correlate in a blockade of memory formation or expression in the humoral anti-H-2^d response.