Inhibition of Adrenal Cholesterogenesis in vitro by AY-9944, 22,25-Diazacholestanol and by Triparanol

Abstract

Summary AY-9944, 22,2 5-diazacholesta-nol and triparanol, known to inhibit hepatic cholesterogenesis, significantly suppressed the incorporation of 2-C¹⁴-acetate into cholesterol by bovine adrenal slices. Thus, by suggesting the presence in beef adrenals of 7-dehydro-cholesterol Δ⁷-reductase and of 24-dehydros-terol Δ²⁴-reductase, the finding indicates a similar pattern of hepatic and adrenal cholesterol synthesis. We wish to thank Mr. A. Smith, Canada Packers for the generous supply of adrenal glands and to Dr. J. P. Giroud, Montreal Children's Hospital for helpful suggestions.