Physical characterization of ilv-lac fusions
- 1 October 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 140 (1), 251-260
- https://doi.org/10.1128/jb.140.1.251-260.1979
Abstract
EM heteroduplex analysis and comparative restriction digests were used to characterize .lambda.p1(209) and .lambda.p123(209), the complementary pair of phages used in the Casadaban technique of gene fusion. Derivatives of .lambda.p1(209) constructed to carry fusions of the lac genes to the control regions of the ilvC and ilvEDA operons were also analyzed. These physical maps provided confirmation of the genetic models for these constructions and physical specifications important in interpreting the behavior of these ilv-lac fusions.This publication has 20 references indexed in Scilit:
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