FLUOROMETRIC DETERMINATION OF MICRO AMOUNTS OF OESTRONE-OESTRADIOL AND OESTRIOL IN URINE
- 1 June 1952
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 10 (2), 149-166
- https://doi.org/10.1530/acta.0.0100149
Abstract
An improved procedure is descr. which uses several ether and NaOH extractions, several dryings and treatments with ethyl alcohol, etc. The final residue is taken up in 85% H3PO4 and read in a Farrand fluorometer. Starting with 10 ml. of urine, recoveries of 80% were obtained, and the test can determine 20-30 [image]g. estrogen/l.Keywords
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