Templated Self-Assembly of a Covalent Polymer Network for Intracellular Protein Delivery and Traceless Release
- 13 April 2017
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 139 (16), 5676-5679
- https://doi.org/10.1021/jacs.7b01214
Abstract
Trafficking proteins inside cells is an emerging field with potential utility in basic cell biology and biological therapeutics. A robust and sustainable delivery strategy demands not only good protection of the cargo but also reversibility in conjugation and activity. We report a protein-templated polymer self-assembly strategy for forming a sheath around the proteins and then tracelessly releasing them in the cytosol. The versatility of the approach, demonstrated here, suggests that the strategy is compatible with a wide array of biologics.Keywords
Funding Information
- Army Research Office (W911NF-15-1-0568)
This publication has 31 references indexed in Scilit:
- Concurrent Binding and Delivery of Proteins and Lipophilic Small Molecules Using Polymeric NanogelsJournal of the American Chemical Society, 2012
- Boronate-Mediated Biologic DeliveryJournal of the American Chemical Society, 2012
- Tailoring nanocarriers for intracellular protein deliveryChemical Society Reviews, 2011
- FDA-approved poly(ethylene glycol)–protein conjugate drugsPolymer Chemistry, 2011
- Protein conjugation of thermoresponsive amine-reactive polymers prepared by RAFTPolymer Chemistry, 2010
- Cytochrome c Encapsulating Theranostic Nanoparticles: A Novel Bifunctional System for Targeted Delivery of Therapeutic Membrane-Impermeable Proteins to Tumors and Imaging of Cancer TherapyMolecular Pharmaceutics, 2010
- Thiol–Ene Click ChemistryAngewandte Chemie-International Edition, 2010
- Synthesis of Aminooxy End-Functionalized pNIPAAm by RAFT Polymerization for Protein and Polysaccharide ConjugationMacromolecules, 2009
- Selectivity in the Post-Translational, Transglutaminase-Dependent Acylation of Lysine ResiduesBiochemistry, 2009
- Protein therapeutics: a summary and pharmacological classificationNature Reviews Drug Discovery, 2008