Comparison of enzyme-multiplied immunoassay technique with fluorescence polarization immunoassay for determination of gentamicin and tobramycin levels in serum

Abstract

Serum gentamicin and tobramycin specimens were assayed by the enzyme multiplied immunoassay technique (Syva EMIT) and the fluorescence polarization immunoassay. When interassay and intraassay control samples were evaluated, both methods gave an overall coefficient of variation of < .+-. 10%. Using patient serum samples, excellent correlation was obtained with both methods in the assay of gentamicin (correlation coefficient, 0.985) and tobramycin (correlation coefficient, 0.982).