INVESTIGATIONS OF A NEW, HIGHLY NEGATIVE LIPOSOME WITH IMPROVED BIODISTRIBUTION FOR IMAGING

Abstract

An attractive feature of liposomes is the wide range of lipid composition that can lead to liposome formation, coupled with the observation that liposome biodistribution may be altered by varying lipid composition. Adding charged lipids to neutral lecithin will alter the biodistribution of the resulting charged liposomes. Highly negative liposomes were prepared by replacing lecithin with negatively charged cardiolipin. The liposomes were labeled in the lipid phase with 67Ga and 99mTc oxine and their properties evaluated. The expected high negative charge of the resulting liposomes was confirmed by an ion-exchange chromatographic technique. Using paper chromatography, the stability of the label was determined during incubation in saline and serum. Finally, biodistributions were determined at 2 h in mice, and the results compared with those for negative lecithin liposomes. Accumulated activities in liver and spleen were reduced by factors of 5 and 20, respectively, over lecithin liposomes. Since preferential accumulation of activity in these organs constitutes the biggest limitation to the use of lecithin liposomes, cardiolipin liposomes may be more useful carriers of radioactivity in imaging applications. Studying novel liposome types as potential radiopharmaceuticals is valuable.