Cytopathogenicity of Mumps Virus in Cultures of Chick Embryo and Human Amnion Cells.

Abstract

Cytopathogenicity of 2 strains of mumps virus for primary cultures of chick embryo (CE) and human amnion (HA) cells was studied. The chick adapted Enders strain induced formation of multinucleated giant cells and extensive cellular destruction in CE cells but did not visibly affect HA cells. Multinucleated giant and spindle-like cells were observed in cultures of HA cells infected with a low passage human amnion adapted strain, which did not visibly affect CE cells. With agar overlay technic viral plaques were obtained with both strains in susceptible cell monolayers.