Activation and Oxidation of Acetic Acid-1-C14 by Cell Free Homogenates of Germinating Peanut Cotyledons
Open Access
- 1 March 1965
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 40 (2), 286-289
- https://doi.org/10.1104/pp.40.2.286
Abstract
A soluble enzyme preparation having acetyl-thiokinase activity is described in homogenates of germinating peanut cotyledons. This enzyme is capable of activating acetic acid to acetyl coenzyme A in the presence of coenzyme A, adenosine triphosphate, and Mg ion. The oxidation of palmitic acid-11-C14 to C14O2 requires the presence of both a particulate fraction sedimenting like mitochondria, and a soluble protein fraction. Likewise synthetic acetyl coenzyme A requires both soluble and particulate components for oxidation of CO2. The extent of this oxidation is increased markedly by addition of glyoxylate.Keywords
This publication has 14 references indexed in Scilit:
- Fractionation and Properties of an Extra-Mitochondrial Enzyme System from Peanuts Catalyzing the β-Oxidation of Palmitic AcidPlant Physiology, 1965
- The Glyoxylate Cycle in Maize ScutellumPlant Physiology, 1964
- Nucleic Acid, Mitochondria, & Enzyme Changes in Cotyledons of Peanut Seeds during GerminationPlant Physiology, 1963
- Metabolic Production of Sucrose from FatNature, 1961
- Influence of Certain Cations on Activity of Acetic Thiokinase from Spinach LeavesPlant Physiology, 1960
- Enzymes of the Glyoxylate Cycle in Germinating Peanuts and Castor BeansJournal of Biological Chemistry, 1960
- FAT METABOLISM IN HIGHER PLANTS .11. CONVERSION OF FAT INTO CARBOHYDRATE IN PEANUT AND SUNFLOWER SEEDLINGS1959
- Fat Metabolism in Higher Plants. VII. β-Oxidation of Fatty Acids by Peanut Mitochondria.Plant Physiology, 1956
- Fat Metabolism in Higher Plants. III. Enzymatic Oxidation of Glycerol.Plant Physiology, 1955
- Acetate activation and acetoacetate formation in plant systemsArchives of Biochemistry and Biophysics, 1954