Increase in CO3H— influx and cellular pH in glucose‐stimulated pancreatic islets

Abstract

When rat islets are preincubated with fluorescein diacetate and examined in a spectrofluorometer, the intracellular pH rapidly increases by 0.21 pH units in response to a rise from 1.7 – 16.8 mM glucose. This coincides with a marked increase in ¹⁴Co₃H^— net uptake by the islets, suggesting that the glucose‐induced increase in H⁺ generation rate is compensated for by stimulation of CO₃ H^—/Cl⁻ exchange.