Relationship between DNA strand breaks and inhibition of poly(ADP-ribosylation): enhancement of carcinogen-induced transformation
- 1 January 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 7 (2), 327-330
- https://doi.org/10.1093/carcin/7.2.327
Abstract
Inhibition of poly(ADP-Rib) by benzamide (BA) or 3-aminobenzamide (3AB) for a limited period (i.e., when ADP-ribosylation is elevated) during and shortly following X-ray or MNNG-induced DNA damage of BALB/3T3 cells significantly (3- to 30-fold) enhanced transformation frequency by these agents. Individual Type III foci isolated from benzamide, X-ray, or X-ray plus benzamide treated cultures were established and characterized for growth in soft agar and for tumor induction in nude mice. DNA isolated from representative transformed lines established as a result of BA, X-ray or X-ray and BA treatments was transfected onto NIH/3T3 cells. Transformation efficiencies ranging from 0.17 to 0.28 foci/ μg of DNA were observed suggesting the possibility that dominant transforming gene(s) were responsible for the oncogenic phenotype of radiation and benzamide transformed DNA.This publication has 3 references indexed in Scilit:
- Enhancement of DEN initiation of liver carcinogenesis by inhibitors of NAD+ ADP ribosyl transferase in ratsCarcinogenesis: Integrative Cancer Research, 1984
- Inhibition of malignant transformation in vitro by inhibitors of poly(ADP-ribose) synthesis.Proceedings of the National Academy of Sciences, 1984
- The Enhancement of Cytotoxicity of N‐Methyl‐N‐nitrosourea and of y‐Radiation by Inhibitors of Poly(ADP‐ribose) PolymeraseEuropean Journal of Biochemistry, 1980