DIFFERENTIATION OF THE ROLES OF HISTAMINE Hr AND H2‐RECEPTORS IN THE MEDIATION OF THE EFFECTS OF HISTAMINE IN THE ISOLATED WORKING HEART OF THE GUINEA‐PIG

Abstract

1 Differentiation of the rôles of histamine H_r and H₂-receptors in the mediation of the effects of histamine on the isolated working heart of the guinea-pig was achieved through the use of histamine and selective histamine receptor agonists and antagonists. 2 Histamine over the dose range 10⁻⁹mol to 10⁻⁶mol produced dose-related increases in sinus rate, left intraventricular pressure (LVP)_max, LVdP/dt_max, coronary flow, aortic flow, total cardiac output and external pressure-volume work. 3 Dimaprit, a selective histamine H₂-receptor agonist, produced very similar responses to histamine. 4 2-Pyridylethylamine, a selective histamine Hj-receptor agonist, had little effect on cardiac function unless large doses were administered. Such doses produced increases in all measured parameters. 5 Cimetidine, a selective histamine H₂-receptor antagonist, antagonized the effects of histamine and dimaprit and some but not all effects of 2-pyridylethylamine. In the presence of cimetidine a decrease in all parameters with the exception of sinus rate was observed with both histamine and 2-pyridylethylamine. 6 The selective histamine Hi-receptor antagonist, mepyramine, had little effect on responses to all three agonists. However, the depressant effects observed with histamine and 2-pyridylethylamine in the presence of cimetidine were antagonized by mepyramine. 7 The results indicate the important rôle of the histamine H₂-receptor in the mediation of the gross cardiac effects of histamine and also indicate that histamine H_x-receptors can mediate cardiac depression.