Elution of Exocellular Enzymes from Saccharomyces fragilis and Saccharomyces cerevisiae
- 1 January 1966
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 91 (1), 1-+
- https://doi.org/10.1128/jb.91.1.1-13.1966
Abstract
Invertase and acid phosphatase are repressible exocellular enzymes in Saccharomyces fragilis and S. cerevisiae. The conditions for eluting these enzymes from both organisms were compared. Either KCl or [beta]-mercaptoethanol eluted the enzyme from S. fragilis, and the amounts eluted varied quantitatively according to the physiological age of the organism. In addition to eluting enzymatic activity from the cells, these reagents also caused a large increase in the amount of activity that remained associated with the cells of S. fragilis. Invertase and acid phosphatase were not removed from the cells of S. cerevisiae by KCl or [beta]-mercaptoethanol. These enzymes were separated from S. cerevisiae cells only when there was some degree of cell-wall digestion by snail gut fluid.This publication has 26 references indexed in Scilit:
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