Expression of Interferon‐α and Interferon‐β Genes in Human Lymphoblastoid (Namalwa) Cells
Open Access
- 1 June 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 133 (2), 399-404
- https://doi.org/10.1111/j.1432-1033.1983.tb07476.x
Abstract
Treatment of human lymphoblastoid (Namalwa) cells with Sendai virus induced the coordinate synthesis of both IFN-α and IFN-β interferon mRNAs. One sub-line of Namalwa cells (WRL) produced no IFN-β activity, although IFN-β mRNA was induced and was associated with polysomes. The IFN-α mRNA was heterogeneous, ranging in size over 1.20–1.35 × 103 bases, probably because of variation in the size of the transcribale DNA in the α-gene family. The IFN-β mRNA was monodisperse with a size of 1.05 × 103 bases. The kinetics of accumulation and decay of both IFN-α and IFN-β mRNAs, as assessed by hybridization with cDNA probes, were very similar to those of translatable interferon mRNA, as assessed by translation in Xenopus oocytes. Treatment of the cells with butyrate or 5′-bromodeoxyuridine increased the amount of hybridizable IFN-α and IFN-β mRNA about 15-fold and 4-fold respectively, again demonstrating coordinate control of IFN-α and IFN-β production.This publication has 30 references indexed in Scilit:
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