Vergleichende Untersuchung der modernen quantitativen Plasma-Fibrinogen-Bestimmungsmethoden

Abstract

A number of methods for the estimation of fibrinogen was investigated. Sensitivity of the individual methods was determined, and the most suitable from each group of methods was selected, and details concerning optimum experimental procedure are given. The methods were: (1) precipitation of fibrinogen followed by gravimetric determination of the precipitate on the micro-balance (method of Morrison); (2) estimation of the precipitated fibrinogen by means of the Biuret reaction according to Mizuta; (3) by means of the tyrosine reaction (method of Ratnoff and Mencie); (4) by means of the Nessler reaction following wet ashing (method of Delory); (5) by means of the ninhydrin reaction (method of Saifer and Newhouse); (6) a nephelometric method (method of Parfentiev et al); and (7) by means of electrophoresis on paper (method of Antonini-Piva). The most exact methods were judged to be those using biuret, ninhydrin and nephelometry.