High Performance Liquid Chromatography of Low Molecular Weight Proteins on a Non-Ionic Macroreticular Polystyrene Resin
- 1 May 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (6), 1101-1115
- https://doi.org/10.1080/01483918408074031
Abstract
A high performance liquid chromatography system is presented for analytical and preparative separation of proteins. The method utilizes a macroreticular polystyrene resin having no specific functional groups, and proteins are eluted by the use of linear gradient of acetonitrile (20-75%, vol/vol) in 0.1% (vol/vol) trifluoroacetic acid. In this standard elution system, 20 proteins having a MW of 4200-58,000 and an isoelectric point of 3.9-11.0 were chromatographed successfully within 80 min. The method allows a rapid, sensitive and high resolution separation of relatively low MW proteins, where the isolated proteins can be used for subsequent biochemical determinations.This publication has 27 references indexed in Scilit:
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