Tumour necrosis factor‐α up‐regulates decay‐accelerating factor gene expression in human intestinal epithelial cells
- 1 March 1997
- journal article
- Published by Wiley in Immunology
- Vol. 90 (3), 358-363
- https://doi.org/10.1111/j.1365-2567.1997.00358.x
Abstract
The increased expression of decay-accelerating factor (DAF) has been detected in intestinal epithelial cells at the inflamed mucosa. In this study, we examined the effects of tumour necrosis factor (TNF)-alpha on DAF expression in three intestinal epithelial cell lines. DAF mRNA expression was evaluated by Northern blot analysis, and DAF protein expression was analysed by biotin labelling and immunoprecipitation. TNF-alpha induced a marked increase in DAF mRNA and protein expression in HT-29, T84 and Caco-2 cells. In HT-29 cells, the effects of TNF-a on DAF mRNA accumulation were observed in a dose-dependent manner; DAF mRNA accumulation reached a maximum at 3-6 hr, and then gradually decreased. These effects of TNF-alpha required de novo protein synthesis. Messenger RNA stability studies suggested that TNF-alpha partially regulated DAF gene expression by a posttranscriptional mechanism. Moreover, the combination of TNF-alpha and interleukin (IL)-4 induced an additive increase in DAF mRNA accumulation in HT-29 and T84 cells. In human intestinal epithelial cells, TNF-alpha acts as a potent inducer of DAF mRNA expression, indicating an important role for TNF-alpha in the regulation of DAF expression at the inflamed mucosa.Keywords
This publication has 30 references indexed in Scilit:
- In Vivo Transfer of GPI-Linked Complement Restriction Factors from Erythrocytes to the EndotheliumScience, 1995
- Complement regulatory molecules: application to therapy and transplantationImmunology Today, 1995
- Enhanced expression of decay accelerating factor and CD59/homologous restriction factor 20 on the colonic epithelium of ulcerative colitis.1995
- Distribution of complement regulatory proteins, decay-accelerating factor, CD59/homologous restriction factor 20 and membrane cofactor protein in human colorectal adenoma and cancer.1994
- Superinduction of cytokine gene transcription by ciprofloxacin.The Journal of Immunology, 1994
- Tumor necrosis factor α-producing cells in the intestinal mucosa of children with inflammatory bowel diseaseGastroenterology, 1994
- Differential cytokine regulation of complement C3, C4, and factor B synthesis in human intestinal epithelial cell line, Caco-2.The Journal of Immunology, 1993
- Decay-accelerating factor (DAF, CD55) in normal colorectal mucosa, adenomas and carcinomasBritish Journal of Cancer, 1992
- Tumour necrosis factor alpha in stool as a marker of intestinal inflammationThe Lancet, 1992
- Wheat germ agglutinin and other selected lectins increase synthesis of decay-accelerating factor in human endothelial cells.The Journal of Immunology, 1991