The specific assay of arylsulphatase C, a rat liver microsomal marker enzyme
- 1 June 1972
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 128 (2), 331-336
- https://doi.org/10.1042/bj1280331
Abstract
Conditions based on previous assays with potassium p-acetylphenyl sulphate have been established for the specific assay of arylsulphatase C in rat tissues. The enzyme has optimum activity with 40mm substrate at pH8.0 in the presence of 0.1m-phosphate buffer. Under these conditions arylsulphatase C can be assayed without interference from the other arylsulphatase enzymes present and is useful as a marker for the endoplasmic reticulum in cell-fractionation studies.Keywords
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