31p nme spectroscopic study of the effects of γ‐irradiation on rif‐1 tumor cells perfused in vitro

Abstract

In order to examine the mechanisms underlying radiation‐induced changes in phosphorus metabolite levels observed in RIF‐1 tumors in vivo, RIF‐1 cells in culture were perfused for up to 70 h following γ‐irradiation with 0‐25 Gy and monitored continuously by, ³¹P NMR spectroscopy at 8.5 T. Cells immobilized in the sample volume by incorporation into calcium alginate beads were bioenergetically stable, but did not replicate at the cell density used. Following an initial increase in PCr and NTP, which occurred in both control and irradiated cells, a dramatic decline in high‐energy phosphates was detected beginning 24‐30 h after irradiation with 15 or 25 Gy. In contrast, unirradiated cells or cells treated with 10 Gy remained metabolically stable for up to 72 h. The metabolic changes induced by irradiation of the cultured cells, which reflected cell death and lysis, were distinctly different from those observed in RIF‐1 tumors in vivo during the same postirradiation time interval‐an increase in high–energy relative to low‐energy phosphates. This suggests that the spectral changes in vivo do not result from direct modification of cellular energy metabolism by radiation injury.