Preparation of Purified Bovine Luteinizing Hormone
- 1 November 1962
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 71 (5), 729-733
- https://doi.org/10.1210/endo-71-5-729
Abstract
A technically simple method is described for the preparation of purified bovine luteinizing hormone. Preparations are attained which contain 1.05 and 1.69 units/mg when assayed in terms of NIH-LH-Sl by the ovarian ascorbic acid depletion assay of Parlow. Thyroid-stimulating hormone contamination is low, and no contamination with follicle- stimulating hormone could be detected when the material was tested at a total dose of 8 mg by the HCG-augmentation method of Steelman and Pohley.Keywords
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