Genome Engineering ofDrosophilawith the CRISPR RNA-Guided Cas9 Nuclease
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Open Access
- 1 August 2013
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 194 (4), 1029-1035
- https://doi.org/10.1534/genetics.113.152710
Abstract
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.Keywords
This publication has 32 references indexed in Scilit:
- One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome EngineeringCell, 2013
- Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryosCell Research, 2013
- Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systemsNucleic Acids Research, 2013
- Multiplex Genome Engineering Using CRISPR/Cas SystemsScience, 2013
- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial ImmunityScience, 2012
- Targeted Genome Editing Across Species Using ZFNs and TALENsScience, 2011
- Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineeringProceedings of the National Academy of Sciences, 2009
- Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleasesProceedings of the National Academy of Sciences, 2008
- A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in DrosophilaProceedings of the National Academy of Sciences, 2008
- Emerging technologies for gene manipulation in Drosophila melanogasterNature Reviews Genetics, 2005