A Soluble Super-Active Form of Insulin
Open Access
- 1 May 1974
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (5), 1630-1633
- https://doi.org/10.1073/pnas.71.5.1630
Abstract
Insulin-like material can be extracted from insulin-Sepharose. This soluble material elicits super-insulin responses from mammary epithelial cells. It stimulates the accumulation of α-aminoisobutyric acid by virgin mouse mammary cells, which are unresponsive to insulin itself. It exerts a greater stimulatory effect on mammary cells from pregnant mice than insulin. It also is more efficacious than insulin as a stimulant of DNA synthesis and the combined activities of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and phosphogluconate dehydrogenase (EC 1.1.1.43). Its specific biological activity on these cells is more than five times greater than that of insulin.Keywords
This publication has 9 references indexed in Scilit:
- Studies on the Mode of Action of Insulin: Properties and Biological Activity of an Insulin-Dextran ComplexEndocrinology, 1972
- Insulin-Sepharose and the Dynamics of Insulin ActionProceedings of the National Academy of Sciences, 1971
- Development of Insulin-Sensitivity by Mouse Mammary Gland In VitroProceedings of the National Academy of Sciences, 1970
- Increase in activity of glucose 6-phosphate dehydrogenase in mouse mammary tissue cultured with insulinBiochemical Journal, 1969
- INTERACTION OF INSULIN WITH THE CELL MEMBRANE: THE PRIMARY ACTION OF INSULINProceedings of the National Academy of Sciences, 1969
- The role of DNA synthesis and mitosis in hormone-dependent differentiation.Proceedings of the National Academy of Sciences, 1966
- Hormone-dependent differentiation of mammary gland in vitro.Proceedings of the National Academy of Sciences, 1965
- Spectrophotometry in the Far UltravioletScience, 1957
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951