Expression of a Gene Cluster kaiABC as a Circadian Feedback Process in Cyanobacteria
- 4 September 1998
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 281 (5382), 1519-1523
- https://doi.org/10.1126/science.281.5382.1519
Abstract
Cyanobacteria are the simplest organisms known to have a circadian clock. A circadian clock gene cluster kaiABC was cloned from the cyanobacterium Synechococcus. Nineteen clock mutations were mapped to the three kai genes. Promoter activities upstream of the kaiA and kaiB genes showed circadian rhythms of expression, and both kaiA andkaiBC messenger RNAs displayed circadian cycling. Inactivation of any single kai gene abolished these rhythms and reduced kaiBC-promoter activity. ContinuouskaiC overexpression repressed the kaiBCpromoter, whereas kaiA overexpression enhanced it. TemporalkaiC overexpression reset the phase of the rhythms. Thus, a negative feedback control of kaiC expression by KaiC generates a circadian oscillation in cyanobacteria, and KaiA sustains the oscillation by enhancing kaiC expression.Keywords
This publication has 33 references indexed in Scilit:
- CYANOBACTERIAL CIRCADIAN RHYTHMSAnnual Review of Plant Physiology and Plant Molecular Biology, 1997
- Circadian orchestration of gene expression in cyanobacteria.Genes & Development, 1995
- A common topology of proteins catalyzing ATP‐triggered reactionsFEBS Letters, 1995
- Circadian Clock Mutants of CyanobacteriaScience, 1994
- Temporal Organization: Reflections of a Darwinian Clock-WatcherAnnual Review of Physiology, 1993
- The GTPase superfamily: conserved structure and molecular mechanismNature, 1991
- The P-loop — a common motif in ATP- and GTP-binding proteinsTrends in Biochemical Sciences, 1990
- The Neurospora clock gene frequency shares a sequence element with the Drosophila clock gene periodNature, 1989
- Restoration of circadian behavioural rhythms by gene transfer in DrosophilaNature, 1984
- Cloning of the luciferase structural genes from Vibrio harveyi and expression of bioluminescence in Escherichia coliBiochemistry, 1984