Immunoprecipitation and class-specific immunofluorescence titration of human serum antibodies to Schistosoma mansoni antigens

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Abstract
Summary Twenty sera of individuals with Schistosoma mansoni infections were examined by means of immunoelectrophoresis, Indirect Fluorescent Antibody (IFA) technique and the Defined Antigen Substrate Spheres (DASS) system. Immunoprecipitins against S. mansoni antigen and against antigen of the intermediate host, Biomphalaria glabrata, were demonstrated. The presence of specific anti-S. mansoni IgA, IgM, IgG and IgE antibodies was identified with the IFA-technique on frozen sections of adult parasites. The DASS-system proved to be more sensitive than the IFA-technique.