Requirement of the Yeast RTH1 5′ to 3′ Exonuclease for the Stability of Simple Repetitive DNA
- 14 July 1995
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 269 (5221), 238-240
- https://doi.org/10.1126/science.7618086
Abstract
Simple repetitive DNA sequences are unstable in human colorectal cancers and a variety of other cancers. Mutations in the DNA mismatch repair genes MSH2, MLH1, and PMS1 result in elevated rates of spontaneous mutation and cause a marked increase in the instability of simple repeats. Compared with the wild type, a null mutation in the yeast RTH1 gene, which encodes a 5' to 3' exonuclease, was shown to increase the rate of instability of simple repetitive DNA by as much as 280 times and to increase the spontaneous mutation rate by 30 times. Epistasis analyses were consistent with the hypothesis that this RTH1-encoded nuclease has a role in the MSH2-MLH-1-PMS1 mismatch repair pathway.Keywords
This publication has 29 references indexed in Scilit:
- Conditional Lethality of Null Mutations in RTH1 That Encodes the Yeast Counterpart of a Mammalian 5′- to 3′-Exonuclease Required for Lagging Strand DNA Synthesis in Reconstituted SystemsPublished by Elsevier ,1995
- Mismatch repair gene defects in sporadic colorectal cancers with microsatellite instabilityNature Genetics, 1995
- DNA Loop Repair by Human Cell ExtractsScience, 1994
- Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair.Genes & Development, 1994
- Mutation of a mutL Homolog in Hereditary Colon CancerScience, 1994
- Mutation in the DNA mismatch repair gene homologue hMLH 1 is associated with hereditary non-polyposis colon cancerNature, 1994
- Clues to the Pathogenesis of Familial Colorectal CancerScience, 1993
- The International Collaborative Group on Hereditary Non-Polyposis Colorectal Cancer (ICG-HNPCC)Diseases of the Colon & Rectum, 1991
- THE CHEMISTRY AND BIOLOGY OF LEFT-HANDED Z-DNAAnnual Review of Biochemistry, 1984
- Is there left-handed DNA at the ends of yeast chromosomes?Nature, 1983