STUDIES ON PEPTIDES .128. APPLICATION OF NEW HETEROBIFUNCTIONAL CROSSLINKING REAGENTS FOR THE PREPARATION OF NEUROKININ (A AND B)-BSA (BOVINE SERUM-ALBUMIN) CONJUGATES
- 1 January 1985
- journal article
- research article
- Vol. 26 (2), 121-129
Abstract
A decapeptide corresponding to the entire amino acid sequence of neurokinin A, a porcine spinal cord peptide, was synthesized in a conventional manner using protecting groups removable by 1 M TFMSA-thioanisole in TFA. The HS-CH2CH2CO group was introduced onto the synthetic neurokinin A by reaction of 3-(S-acetyl-thiopropionyl)-thiazolidine-2-thione, followed by deacetylation with hydroxylamine. 2,4-Dinitrophenyl-p-(.beta.-nitrovinyl)-benzoate trapped the above HS-CH2-CH2-CO-neurokinin A derivative in acidic media, then BSA in basic media in nearly quantitative yield. A similar decapeptide, neurokinin B, was also synthesized and conjugated onto BSA using an alternative SH-introducing reagent, 3-(S-p-methoxybenzyl-thiopropionyl)-thiazolidine-2-thione, and the above heterobifunctional conjugating reagent.This publication has 12 references indexed in Scilit:
- A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassayAnalytical Biochemistry, 1983
- Neuromedin K: A novel mammalian tachykinin identified in porcine spinal cordBiochemical and Biophysical Research Communications, 1983
- An immunohistochemical study of neuronal populations containing neuropeptides or γ-aminobutyrate within the superficial layers of the rat dorsal hornNeuroscience, 1981
- Studies on peptides. 103. Chemical synthesis of a crystalline protein with the full enzymic activity of ribonuclease AJournal of the American Chemical Society, 1981
- Total synthesis of bovine pancreatic ribonuclease A. Part 1. Synthesis of the protected pentadecapeptide ester (positions 110–124)Journal of the Chemical Society, Perkin Transactions 1, 1981
- STUDIES ON PEPTIDES .87. SYNTHESIS OF AN OCTACOSAPEPTIDE AMIDE CORRESPONDING TO THE ENTIRE AMINO-ACID-SEQUENCE OF CHICKEN VASOACTIVE INTESTINAL POLYPEPTIDE (VIP)1980
- SIDE REACTIONS IN PEPTIDE-SYNTHESIS .9. SUPPRESSION OF THE FORMATION OF AMINOSUCCINYL PEPTIDES WITH ADDITIVES1978
- SIDE REACTIONS IN PEPTIDE-SYNTHESIS .7. SEQUENCE DEPENDENCE IN FORMATION OF AMINOSUCCINYL DERIVATIVES FROM BETA-BENZYL-ASPARTYL PEPTIDES1978
- Studies on peptides. LXXVI. Synthesis of kassinin, a new frog skin peptide.CHEMICAL & PHARMACEUTICAL BULLETIN, 1978
- Une nouvelle méthode de synthèse peptidiqueHelvetica Chimica Acta, 1951