Isolated Nephron Segments from Rabbit Models of Obstructive Nephropathy

Abstract

Micropuncture and microcatheterization studies have been used extensively to investigate the pathophysiologic alterations in renal function induced by urinary tract obstruction. The present isolated tubule microperfusion studies were designed to examine the intrinsic alterations in segmental nephron function induced by 24 h of bilateral (BUO) and unilateral (UUO) urinary tract obstruction. Following UUO superficial proximal convoluted tubule reabsorption rate (J_v) was not different from contralateral control (0.75±0.08 vs. 0.73±0.11 nl/mm per min, NS). Following UUO J_v in juxtamedullary proximal convoluted tubules (JMPCT) was reduced 32% (0.69±0.06 vs. 0.47±0.04 nl/mm per min, P < 0.02). Following UUO J_v in proximal straight tubules (PST) was reduced 52% (0.25±0.02 vs. 0.12±0.03, P < 0.01). Thick ascending limb (T-ALH) function was assessed by measurement of ability to lower perfusate chloride ion concentration (ΔCl). Following UUO ΔCl was reduced 76% (−39±9 vs. −9±1 meq/liter, P < 0.001). Cortical collecting tubule (CCT) function was assessed by measurement of antiduiretic hormone (ADH)-dependent osmotic water flow. Following UUO osmotic water flow was reduced 76% (0.90±0.08 vs. 0.22±0.04 nl/mm per min, P < 0.01) and this ADH resistance could not be overcome by cAMP. Nephron segments were then examined following relief of BUO. There were no differences in intrinsic function following relief of BUO when compared with UUO. We conclude that in UUO and BUO (a) the intrinsic tubular defects are identical, (b) the natriuresis noted is due, in part, to disordered JMPCT, PST, and T-ALH NaCl reabsorption, (c) the impaired concentrating ability is due, in part, to depressed function in T-ALH and ADH resistance of the CCT, and (d) the ADH resistance occurs at a site distal to the intracellular generation of cAMP.