DNA Binding Activity of Recombinant SRY from Normal Males and XY Females
- 24 January 1992
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 255 (5043), 453-456
- https://doi.org/10.1126/science.1734522
Abstract
The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence AACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.Keywords
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