A mathematical analysis of enzyme stabilization by a series‐type mechanism: Influence of chemical modifiers

Abstract

A series-type enzyme deactivation model is utilized to theoretically analyze and to quantify the effect of chemical modifier concentration on the eventual level of enzyme activity stabilization, α₂. An increase in the concentration of phosphate ion and NADP increases α₂ for the enzymes studied. One example of each enzyme deactivation is given wherein the introduction of chemical modifiers changes the deactivation mechanism from a single-step to a series-type mechanism, and from a series-type to a single-step mechanism. Simple empirical equations are proposed to quantify the effect of chemical modifier concentration on α₂.