Properties of Antibodies to Thymine Glycol, a Product of the Radiolysis of DNA
- 1 March 1984
- journal article
- research article
- Published by JSTOR in Radiation Research
- Vol. 97 (3), 499-510
- https://doi.org/10.2307/3576140
Abstract
Anti-thymine glycol antibodies were elicited by immunizing rabbits with thymine glycol monophosphate (TMP-glycol) conjugated by carbodiimide to BSA [bovine serum albumin]. The antibodies produced are specific for thymine glycol as measured by immunoprecipitation of TMP-glycol-RSA [rabbit serum albumin] conjugates and hapten inhibition of reactivity with OsO4-treated DNA in an enzyme immunoassay. Using the enzyme immunoassay, the antibody is capable of detecting femtomole and picomole levels of thymine glycol in direct and competitive assays, respectively. This immunochemical assay is potentially suitable for measuring the production and repair of thymine glycol damage in cellular DNA.This publication has 15 references indexed in Scilit:
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