A quantitative histologic analysis of the growing long bone metaphysis
- 1 December 1980
- journal article
- research article
- Published by Springer Nature in Calcified Tissue International
- Vol. 32 (1), 113-122
- https://doi.org/10.1007/bf02408530
Abstract
The purpose of this work was to analyze the proximal tibial metaphysis of the 170 g rat in a quantitative histologic fashion which would allow some relation to tissue age to be established. Stained 3 µm thick tissue sections were analyzed with the aid of a Merz grid on an eyepiece reticule and a light microscope. Tissue mass and cell distribution were studied in all areas. The rate of change in tissue mass during aging of the metaphysis was calculated. Two regions of the metaphysis were identified. One, corresponding to the primary spongiosa, less than 4.45 days of age, is a region of high turnover of hard tissue and high numbers of osteoblasts and osteoprogenitor cells. The other, corresponding to the secondary spongiosa, is a region of relatively low net tissue turnover and low numbers of osteoblasts and osteoprogenitor cells. Osteoclasts were found relatively more uniformly distributed through the metaphysis than were osteoblasts and osteoprogenitor cells. The rate of bone formation in the primary spongiosa is 50 times that found in the Haversian bone of the rib of 5-year-old humans and about 500 times that found at the cortical-endosteal surface of ribs of 5-year-old humans. It is argued that both cell distribution and tissue distribution in the metaphysis support the concept that osteoblasts and osteoclasts, rather than osteocytes, are responsible for the maturation of the metaphysis. The inhomogeneous distribution of both cells and tissue in the metaphysis has definite meaning for the interpretation of findings concerning the incorporation of radionuclides into the skeleton.Keywords
This publication has 15 references indexed in Scilit:
- Osteoclast cell-surface changes during the egg-laying cycle in Japanese quailThe Journal of cell biology, 1977
- Skeletal Changes and Growth in Experimental UremiaNephron, 1977
- CALCITONIN, PARATHYROIDECTOMY AND MODELLING OF BONES IN THE GROWING RATJournal of Endocrinology, 1975
- A Rapid Plastic Embedding Technique for Preparation of Three-Micron Thick Sections of Decalcified Hard TissueStain Technology, 1975
- Effect of ethane-1-hydroxy-1,1-diphosphonate (EHDP) and dichloromethylene diphosphonate (Cl2MDP) on the calcification and resorption of cartilage and bone in the tibial epiphysis and metaphysis of ratsCalcified Tissue International, 1973
- MECHANISMS OF BONE RESORPTION IN HUMAN METAPHYSEAL REMODELINGAmerican Journal of Roentgenology, 1971
- A quantitative histological study on bone formation in human cancellous boneCells Tissues Organs, 1970
- Tetracycline-based histological analysis of bone remodelingCalcified Tissue International, 1969
- Effect of Hydrocortisone on the Utilization of Tritiated Thymidine for Skeletal Growth in the RatAnnals Of The Rheumatic Diseases, 1964
- CELL PROLIFERATION AND SPECIALIZATION DURING ENDOCHONDRAL OSTEOGENESIS IN YOUNG RATSThe Journal of cell biology, 1962