Apparatus and technique for two-dimensional paper ionophoresis

Abstract

In a new apparatus for two-dimensional paper ionophoresis, the pH of the buffer solution in the paper is changed, after ionophoresis in one dimension, by exposure to the vapor from a-solution exerting a suitable and substantially constant partial pressure of ammonia. A method for determining the flow of electrolyte at different points on the paper is proposed. The results of applying it in the new apparatus are discussed. The following amino acids are separated qualitatively by the new apparatus: glutamic and aspartic acids, cystine, glycine, alanine, valine, leucine, phenylalanine, serine, threonine, tyrosine, hydroxyproline, proline, histidine, lysine and arginine. Leucine and isoleucine are not separated. The developed ionophoretogram is ready within 9 hours of starting an experiment.