Cooperative tandem binding of met repressor of Escherichia coli
- 1 October 1989
- journal article
- Published by Springer Nature in Nature
- Vol. 341 (6244), 711-715
- https://doi.org/10.1038/341711a0
Abstract
We present biochemical and genetic data to support the hypothesis that the Escherichia coli met repressor, MetJ, binds to synthetic and natural operator sequences in tandem arrays such that repression depends not only on the affinity of the DNA-protein interaction, but also on protein-protein contacts along the tandem array. This represents a novel form of regulatory switch. Furthermore, there seems to be homology between the organization of the met and trp operators.Keywords
This publication has 21 references indexed in Scilit:
- Three-dimensional crystal structures of Escherichia coli met repressor with and without corepressorNature, 1989
- How eukaryotic transcriptional activators workNature, 1988
- Gel retardation at low pH resolves trp repressor-DNA complexes for quantitative study.Proceedings of the National Academy of Sciences, 1988
- Nucleotide sequence of the Salmonella typhimurium metR gene and the metR-metE control regionJournal of Bacteriology, 1987
- The crystal structure of trp aporepressor at 1.8 Å shows how binding tryptophan enhances DNA affinityNature, 1987
- Evolution in biosynthetic pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region.Proceedings of the National Academy of Sciences, 1986
- Regulation of methionine synthesis in Escherichia coli : Effect of metJ gene product and S -adenosylmethionine on the expression of the metF geneProceedings of the National Academy of Sciences, 1985
- Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresisNucleic Acids Research, 1981
- Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.Proceedings of the National Academy of Sciences, 1980
- DNAase footprinting a simple method for the detection of protein-DNA binding specificityNucleic Acids Research, 1978