Development of the antibody response in acute HIV-1 infection
- 1 February 2004
- journal article
- basic science
- Published by Wolters Kluwer Health in AIDS
- Vol. 18 (3), 371-381
- https://doi.org/10.1097/00002030-200402200-00002
Abstract
Background: Cytotoxic T lymphocytes have been shown to reduce viraemia during acute HIV-1 infection; however the role of neutralizing antibodies in this process is unclear. One confounding factor may be artefacts introduced by viral culture. Objective: To assess the development of autologous neutralizing and non-neutralizing antibodies following acute HIV-1 infection using recombinant viruses with envelopes amplified directly from patient peripheral blood mononuclear cells, thereby avoiding in vitro selection. Methods: Disease progression in four homosexual men was monitored from acute infection for up to 2.5 years, in the absence of antiretroviral therapy. Antibodies to viral envelope protein were quantified by enzyme-linked immunosorbent assay. Development of neutralizing antibodies was monitored using a quantitative infectivity reduction assay, sequential serum, recombinant viruses and target cells with defined receptor expression. Results: The time to development of neutralizing antibodies after onset of symptoms was 3, 5, 7 and 16 months in the four patients. There was no correlation between development of neutralizing antibodies and the resolution of viraemia in any of the patients. However, antibodies to the envelope were detectable as early as 2 weeks after onset of symptoms. Conclusions: Neutralizing antibodies do not contribute to the control of viraemia in acute HIV-1 infection. However, antibodies to the envelope could be detected at the time of reduction in plasma viraemia and so other effector functions of antibodies may play a role in viral clearance.Keywords
This publication has 57 references indexed in Scilit:
- Antibody neutralization and escape by HIV-1Nature, 2003
- Relationship between Antibody-Dependent Cellular Cytotoxicity, Plasma HIV Type 1 RNA, and CD4+Lymphocyte CountAIDS Research and Human Retroviruses, 2001
- Appearance of Autologous Neutralizing Antibody Correlates with Reduction in Virus Load and Phenotype Switch during Primary Infection with Human Immunodeficiency Virus Type 1The Journal of Infectious Diseases, 1997
- Complement Activation by HIV-1-Infected Target Cells Enhances IL-2-Stimulated but Not Unstimulated ADCC Activity Mediated by peripheral Blood Mononuclear CellsClinical Immunology and Immunopathology, 1996
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Frequent and Early HIV-1MNNeutralizing Capacity in Sera from Dutch HIV-1 Seroconverters Is Related to Antibody Reactivity to Peptides from the gp120 V3 DomainAIDS Research and Human Retroviruses, 1994
- SHORT COMMUNICATIONAIDS, 1991
- Evaluation of Human and Simian Immunodeficiency Virus Plaque and Neutralization AssaysJournal of General Virology, 1989
- Biological significance of the antibody response to HIV antigens expressed on the cell surfaceArchiv für die gesamte Virusforschung, 1988
- Characterization of Serum Netralization Response to the Human Immunodeficiency Virus (HIV)AIDS Research and Human Retroviruses, 1987