Serum Lipid Analyses in Rats Fed Natural and Hydrogenated Cottonseed Oil with Cholesterol and Cholate.

Abstract

Hydrogenated and unhydrogenated cottonseed oil of differing I numbers were used. Three diets were fed, one diet with each fat was also fed with added sodium cholate and/or cholesterol. Dietary cholate with cholesterol added to natural and hydrogenated cottonseed oil raised serum cholesterol and phospholipid. This effect was augmented with saturated fat. Dietary lipid saturation or cholate alone had no effect on serum cholesterol. Cholesterol to phospholipid ratio was elevated only when both dietary cholate and cholesterol were added to hydrogenated fat. In the rat highly saturated fat (I no. 43) fed with cholesterol reverses the alpha to beta lipoprotein (LP) ratio with beta LP becoming dominant. Combination of dietary cholate with cholesterol permits reversal of alpha to beta LP with moderate (I no. 78) or no hydrogenation (I no. 107) of dietary fat. Serum cholesterol ester fatty acid composition changes with greater saturation of dietary fat and added cholate and cholesterol. High oleic acid levels were noted in serum cholesterol ester fraction of rats fed saturated fat (I no. 78) with cholate and cholesterol.