Studies in detoxication

Abstract

The fate of p-hydroxybenzenesulphonamide (phenol-p-sulphonamide) in the rabbit was studied. At a dose level of 0.75 g./kg., about 50% of the dose is excreted conjugated with glucuronic acid, about 30% with sulphuric acid and a small amount is oxidised to a catechol derivative, probably catecholsulphon-amide. No free phenolsulphonamide was excreted. A study of the ethereal sulphate conjugation of phenolsulphonamide at various dose levels showed that the % conjugated fell with increasing dose. The conjugated glucuronide of phenolsulphonamide was isolated as a Ba salt, [[alpha]]n-50[degree], and characterised by means of the crystalline amide of the 2:3:4-trimethyl p-sulphondimethylamidophenyl-[beta]-d-glu-curonide, M.P. 154-5[degree]C. Phenol-p-sul phonic acid, 1-hydroxy-2-amino-, l-amino-2-hydroxy- and l-amino-3-hydroxy-benzene-4-sulphonic acids evidently do not conjugate with glucuronic and sulphuric acids in the rabbit and are probably excreted unchanged.