Separation of Tocols by HPLC on an Amino-Cyano Polar Phase Column
- 1 March 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 8 (4), 707-717
- https://doi.org/10.1080/01483918508067114
Abstract
A method is described for separating α-, β-, γ and δ-tocopherols, their corresponding tocoraonoenols and tocotrienols, and plastochromanol-8 on a 5 μm amino-cyano HPLC column using hexane:tetrahydrofuran (94:6) as eluant. The detection limit for α-tocopherol using fluorescence excitation at 210 nm was 1 ng. Results of analysis on six seed oils are presented.Keywords
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