Reaginic antibody production in different mouse strains.

Abstract

Mice of several strains were immunized with DNP-haemocyanin either with Al(OH)3 or with Freund's complete adjuvant. Antibodies of immunoglobulin type γ2 and γM were detected by passive haemolysis, γ2a antibodies by PCA in guinea-pigs, γ1 antibodies by PCA in mice using 2 hours of SP, and reaginic antibodies by PCA in mice using 72 hours of SP. Three of the six strains tested produced reaginic antibodies, i.e. SWR/J, C57BL/6J and A/HeJ, but only when Al(OH)3 was used as adjuvant. All six strains produced γ1 antibodies. Reaginic antibodies were separated by chromatography on DEAE-cellulose column.