Quantitative Studies on 2 Anti-Dinitrophenyl Antibodies.

Abstract

Summary Guinea pig γ₂ anti-dinitrophenyl antibodies can lyse tanned sheep erythrocytes coated with highly derivatized dinitrophenyl bovine serum albumin. With the method used, 0.3 to 0.6 γ antibody protein/ml is sufficient to provoke lysis. Since no difference was found between high and low affinity anti-bodies, this method can be used for the quan-titation of guinea pig γ₂ anti-dinitrophenyl antibodies of high and/or low affinities.