Synthesis and physical characterization of the self-complementary, alternating pyrimidine/purine hexanucleotide d[CGTACG]
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (20), 6265-6271
- https://doi.org/10.1093/nar/10.20.6265
Abstract
The hexanucleotide d(CGTACG) has been synthesized by a phosphotriester method in which, for the first time, an 0-6 protected deoxyguanosine derivative has been used to avoid side reactions of the guanine residues. Conformational analysis by circular dichroism shows that d(CGTACG) maintains a B form under conditions (5 M NaCl) where the all C/G hexanucleotide d(CG)3 adopts a Z form, even though d(CGTACG) is a fully alternating pyrimidine/purine molecule. The ΔH for the helix-to-coil transition has been measured.Keywords
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