Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity
- 1 August 1980
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 35 (2), 374-381
- https://doi.org/10.1111/j.1471-4159.1980.tb06274.x
Abstract
Microvessels were isolated from rat brain using a double collagenase treatment which removed the endothelial basement membranes. The isolate was characterized by intact luminal and abluminal membranes and an absence of pericytes and astrocyte membranes. Minimal contamination by 5′-nucleotidase, an enzyme believed exclusively localized within the plasma membranes of neuroglia, established the purity of the isolated microvessels. Enrichment of alkaline phosphatase and -γ-glutamyl transpeptidase activity in microvessel preparations supports the endothelial localization of these enzymes.Keywords
This publication has 27 references indexed in Scilit:
- TRANSPORT OF SUGARS INTO MICROVESSELS ISOLATED FROM RAT BRAIN: A MODEL FOR THE BLOOD‐BRAIN BARRIER1Journal of Neurochemistry, 1979
- Isolation from bovine brain of a fraction containing capillaries and a fraction containing membrane fragments of the choroid plexusJournal of Neurobiology, 1976
- The isolation and culture of capillary endothelium from epididymal fatMicrovascular Research, 1975
- Isolation of metabolically active capillaries from rat brain1,2Journal of Neurochemistry, 1975
- Isolated Brain Microvessels: A Purified, Metabolically Active Preparation from Bovine Cerebral CortexScience, 1974
- γ-Glutamyl Transpeptidase in Brain Capillaries: Possible Site of a Blood-Brain Barrier for Amino AcidsScience, 1974
- HUMAN VASCULAR ENDOTHELIAL CELLS IN CULTUREThe Journal of cell biology, 1974
- Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIAJCI Insight, 1973
- Purification and characterization of
(Na . II. Preparation by zonal centrifugation of highly active(Na from the outer medulla of rabbit kidneysBiochimica et Biophysica Acta (BBA) - Biomembranes, 1971 - A low-viscosity epoxy resin embedding medium for electron microscopyJournal of Ultrastructure Research, 1969