SEROTONIN STIMULATES INDEPENDENTLY THE ADHESION AND GROWTH OF THE MULTIPLICATION OF FIBROBLASTS IN CULTURE

Abstract

The influence of serotonin on the adhesion and growth processes in primary cultures of murine and human embryo fibroblasts and mouse neoplastic fibroblast L-cells was studied separately. At the moment of seeding, serotonin shortened the time of adhesion to glass surface of these from .apprx. 4 to 1 or 2 h and stimulated incorporation of [3H] thymidine and cell multiplication when introduced in 2 or 6 h cultures. Adhesion and growth stimulatory activities were blocked by serotonin antagonists, cyproheptadine and tipindole, demonstrating that the specific binding of bioamine with cell receptors is essential in both cases.